Rabies Viral Vectors for Monosynaptic Tracing and Targeted Transgene Expression in Neurons
- 1McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;
- 2Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Abstract
Deletion-mutant rabies viral (RV) vectors are powerful tools for neuroscience, allowing monosynaptic tracing of inputs to defined populations and rapid, high-level transgene expression in neurons targeted by multiple routes. High titers and high purity are critical for the successful use of RV vectors in vivo. Here we present a protocol for producing high-quality viral stocks that can be concentrated by ultracentrifugation for final titers in excess of 1010 infectious units per milliliter.
Footnotes
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↵3 Correspondence: wickersham{at}mit.edu
- © 2015 Cold Spring Harbor Laboratory Press