Breast cancer diagnosis using spatial light interference microscopy

Hassaan Majeed; Mikhail E. Kandel; Kevin Han; Zelun Luo; Virgilia Macias; Krishnarao V. Tangella; Andre Balla; Gabriel Popescu

doi:10.1117/1.JBO.20.11.111210

20 August 2015 Breast cancer diagnosis using spatial light interference microscopy

Hassaan Majeed, Mikhail E. Kandel, Kevin Han, Zelun Luo, Virgilia Macias, Krishnarao V. Tangella, Andre Balla, Gabriel Popescu

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Journal of Biomedical Optics, Vol. 20, Issue 11, 111210 (August 2015). https://doi.org/10.1117/1.JBO.20.11.111210

Abstract

The standard practice in histopathology of breast cancers is to examine a hematoxylin and eosin (H&E) stained tissue biopsy under a microscope to diagnose whether a lesion is benign or malignant. This determination is made based on a manual, qualitative inspection, making it subject to investigator bias and resulting in low throughput. Hence, a quantitative, label-free, and high-throughput diagnosis method is highly desirable. We present here preliminary results showing the potential of quantitative phase imaging for breast cancer screening and help with differential diagnosis. We generated phase maps of unstained breast tissue biopsies using spatial light interference microscopy (SLIM). As a first step toward quantitative diagnosis based on SLIM, we carried out a qualitative evaluation of our label-free images. These images were shown to two pathologists who classified each case as either benign or malignant. This diagnosis was then compared against the diagnosis of the two pathologists on corresponding H&E stained tissue images and the number of agreements were counted. The agreement between SLIM and H&E based diagnosis was 88% for the first pathologist and 87% for the second. Our results demonstrate the potential and promise of SLIM for quantitative, label-free, and high-throughput diagnosis.

Majeed, Kandel, Han, Luo, Macias, Tangella, Balla, and Popescu: Breast cancer diagnosis using spatial light interference microscopy

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Hassaan Majeed, Mikhail E. Kandel, Kevin Han, Zelun Luo, Virgilia Macias, Krishnarao V. Tangella, Andre Balla, and Gabriel Popescu "Breast cancer diagnosis using spatial light interference microscopy," Journal of Biomedical Optics 20(11), 111210 (20 August 2015). https://doi.org/10.1117/1.JBO.20.11.111210

Published: 20 August 2015

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