Abstract
Heterologous endo-xanthanase (EX) from the thermophilic planktomycete Thermogutta terrifontis strain was obtained using Penicillium verruculosum 537 (ΔniaD) expression system with the cellobiohydrolase 1 gene promoter. Homogeneous EX with a molecular weight of 23.7 kDa (pI 6.5) was isolated using liquid chromatography methods. This xanthan degrading enzyme also possesses the enzymatic activity towards CM-cellulose, β-glucan, curdlan, lichenan, laminarin, galactomannan, xyloglucan but not towards p-nitrophenyl derivatives of β-D-glucose, mannose and cellobiose. The temperature and pH optima of EX were 55°C and 4.0, respectively; the enzyme exhibited 90% of its maximum activity in the temperature range 50-60°C and pH 3-5.
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Abbreviations
- CF:
-
culture fluid
- EP:
-
enzyme preparation
- EX:
-
endo-xanthanase
- pNP:
-
para-nitrophenyl derivatives
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This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation (GZ 0104-2019-0009). The work was carried out using scientific equipment of the Center for Collective Use “Industrial Biotechnology” and the Center for Collective Use “Bioengineering” of the Federal Research Center of Biotechnology of the Russian Academy of Sciences. The authors declare no conflict of interest in financial or any other sphere. This article does not contain any studies with human participants or animals performed by any of the authors.
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This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation (GZ 0104-2019-0009). The work was carried out using scientific equipment of the Center for Collective Use “Industrial Biotechnology” and the Center for Collective Use “Bioengineering” of the Federal Research Center of Biotechnology of the Russian Academy of Sciences. The authors declare no conflict of interest in financial or any other sphere. This article does not contain any studies with human participants or animals performed by any of the authors.
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Published in Russian in Biokhimiya, 2021, Vol. 86, No. 4, pp. 575-583, https://doi.org/10.31857/S0320972521040096.
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Denisenko, Y.A., Korotkova, O.G., Zorov, I.N. et al. Heterologous Expression of Thermogutta terrifontis Endo-Xanthanase in Penicillium verruculosum, Isolation and Primary Characterization of the Enzyme. Biochemistry Moscow 86, 489–495 (2021). https://doi.org/10.1134/S000629792104009X
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DOI: https://doi.org/10.1134/S000629792104009X