Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan

Kazuya Shirato; Naganori Nao; Harutaka Katano; Ikuyo Takayama; Shinji Saito; Fumihiro Kato; Hiroshi Katoh; Masafumi Sakata; Yuichiro Nakatsu; Yoshio Mori; Tsutomu Kageyama; Shutoku Matsuyama; Makoto Takeda

doi:10.7883/yoken.JJID.2020.061

Short Communications

Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan

Kazuya Shirato, Naganori Nao, Harutaka Katano, Ikuyo Takayama, Shinji Saito, Fumihiro Kato, Hiroshi Katoh, Masafumi Sakata, Yuichiro Nakatsu, Yoshio Mori, Tsutomu Kageyama, Shutoku Matsuyama, Makoto Takeda

Author information

Kazuya Shirato
Department of Virology III, National Institute of Infectious Disease, Japan
Naganori Nao
Department of Virology III, National Institute of Infectious Disease, Japan
Harutaka Katano
Department of Pathology National Institute of Infectious Disease, Japan
Ikuyo Takayama
Influenza Virus Research Center, National Institute of Infectious Disease, Japan
Shinji Saito
Influenza Virus Research Center, National Institute of Infectious Disease, Japan
Fumihiro Kato
Department of Virology III, National Institute of Infectious Disease, Japan
Hiroshi Katoh
Department of Virology III, National Institute of Infectious Disease, Japan
Masafumi Sakata
Department of Virology III, National Institute of Infectious Disease, Japan
Yuichiro Nakatsu
Department of Virology III, National Institute of Infectious Disease, Japan
Yoshio Mori
Department of Virology III, National Institute of Infectious Disease, Japan
Tsutomu Kageyama
Influenza Virus Research Center, National Institute of Infectious Disease, Japan
Shutoku Matsuyama
Department of Virology III, National Institute of Infectious Disease, Japan
Makoto Takeda
Department of Virology III, National Institute of Infectious Disease, Japan

Keywords: novel coronavirus 2019, nCoV-2019, nested RT-PCR, real-time RT-PCR

JOURNAL FREE ACCESS

2020 Volume 73 Issue 4 Pages 304-307

DOI https://doi.org/10.7883/yoken.JJID.2020.061

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Abstract

During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan.

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