Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors

Immunity. 1999 Jul;11(1):67-77. doi: 10.1016/s1074-7613(00)80082-5.

Abstract

Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell-cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Antigens, Ly*
  • Carrier Proteins / metabolism
  • Cell Adhesion / genetics
  • Cell Adhesion / immunology
  • H-2 Antigens / metabolism
  • Histocompatibility Antigens Class I / genetics
  • Histocompatibility Antigens Class I / metabolism*
  • Immunosuppressive Agents / metabolism*
  • Immunosuppressive Agents / pharmacology
  • Killer Cells, Natural / immunology*
  • Killer Cells, Natural / metabolism*
  • Lectins, C-Type
  • Lymphocyte Culture Test, Mixed
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Membrane Glycoproteins / pharmacology
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Congenic
  • Mice, Inbred A
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Peptides / metabolism
  • Protein Binding / genetics
  • Protein Binding / immunology
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Receptors, Immunologic / physiology
  • Receptors, NK Cell Lectin-Like
  • Solubility

Substances

  • Antigens, Ly
  • Carrier Proteins
  • H-2 Antigens
  • H-2K(K) antigen
  • Histocompatibility Antigens Class I
  • Immunosuppressive Agents
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Membrane Proteins
  • Peptides
  • Receptors, Immunologic
  • Receptors, NK Cell Lectin-Like