Abstract
We investigated the EGL-30 (Gqalpha) pathway in C. elegans by using genetic screens to identify genes that confer phenotypes similar to egl-30 mutants. One such gene, egl-8, encodes a phospholipase Cbeta that is present throughout the nervous system and near intestinal cell junctions. EGL-30 and EGL-8 appear to positively regulate synaptic transmission because reducing their function results in strong aldicarb resistance and slow locomotion rates. In contrast, GOA-1 (Goalpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate synaptic transmission, because reducing their function results in strong aldicarb hypersensitivity and hyperactive locomotion. A genetic analysis suggests that GOA-1 negatively regulates the EGL-30 pathway and that DGK-1 antagonizes the EGL-30 pathway.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans / physiology*
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Diacylglycerol Kinase / physiology*
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GTP-Binding Protein alpha Subunits, Gi-Go
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Helminth Proteins / genetics
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Helminth Proteins / physiology
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Heterotrimeric GTP-Binding Proteins / metabolism
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Heterotrimeric GTP-Binding Proteins / physiology*
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Intestinal Mucosa / metabolism
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Intestines / cytology
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Isoenzymes / genetics
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Molecular Sequence Data
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Mutation / physiology
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Nervous System / cytology
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Nervous System / metabolism
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Phenotype
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Phospholipase C beta
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Synaptic Transmission / physiology
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Type C Phospholipases / genetics
Substances
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Helminth Proteins
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Isoenzymes
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Diacylglycerol Kinase
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Type C Phospholipases
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Phospholipase C beta
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GTP-Binding Protein alpha Subunits, Gi-Go
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Heterotrimeric GTP-Binding Proteins