Abstract
The activation of many genes requires the concerted effort of two or more transcription factors. Although C/EBP beta is known to cooperate with Myb to induce transcription of the granulocyte-specific mim-1 gene, the molecular mechanism of this cooperativity is undefined. We show that the N terminus of the full-length C/EBP beta isoform, which is essential for induction of the mim-1 gene in chromatin, interacts specifically with the SWI/SNF complex. Grafting this domain onto Myb generates a chimeric activator that recruits SWI/SNF and induces mim-1 transcription in the absence of C/EBP beta. Interaction between C/EBP beta and SWI/SNF is essential for activating a subgroup of resident target genes in chromatin and may represent a major determinant of combinatorial gene regulation in eukaryotes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases*
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Adenosine Triphosphatases
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Animals
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Binding Sites
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CCAAT-Enhancer-Binding Proteins
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Cell Cycle Proteins*
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Cell Line
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Chromatin / genetics
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Chromatin / metabolism
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DNA Helicases
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Drosophila Proteins
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Erythroblasts / cytology
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Erythroblasts / metabolism
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Fibroblasts / cytology
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Fibroblasts / metabolism
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Humans
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Multienzyme Complexes / metabolism*
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Promoter Regions, Genetic / genetics
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Protein Binding
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Protein Isoforms / chemistry
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Protein Isoforms / metabolism
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Proteins / genetics*
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Proto-Oncogene Proteins c-myb / genetics
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Proto-Oncogene Proteins c-myb / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins
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Sequence Deletion / genetics
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation / genetics*
Substances
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CCAAT-Enhancer-Binding Proteins
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Cell Cycle Proteins
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Chromatin
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DNA-Binding Proteins
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Drosophila Proteins
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Multienzyme Complexes
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Nuclear Proteins
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Protein Isoforms
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Proteins
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Proto-Oncogene Proteins c-myb
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Recombinant Fusion Proteins
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SMARCA1 protein, human
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SMARCA2 protein, human
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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brm protein, Drosophila
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Acetyltransferases
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mim-1 protein, Gallus gallus
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Adenosine Triphosphatases
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SMARCA4 protein, human
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SNF2 protein, S cerevisiae
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DNA Helicases