Abstract
Using immunoglobulin heavy chain transgenic mice, we show that B cell clones reaching the long-lived pool are heterogeneous: some are enriched in the CD21(high) compartment (mostly marginal zone [MZ]), others reside primarily in the follicles (FO). Altering the composition of the B cell receptor through N region additions decreases the rate of clonal production and the MZ enrichment. This process can be recapitulated by purified CD21(low) B cells and is due to a preferential clonal survival that requires a functional btk tyrosine kinase. We also show that generation of the MZ population is dependent on CD19. These findings suggest that the MZ B cell repertoire is positively selected and have functional implications for antigenic responses effected by B cells from this microenvironment.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Agammaglobulinaemia Tyrosine Kinase
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Animals
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Antigens, CD19 / genetics
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Antigens, CD19 / metabolism
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Antigens, CD19 / physiology*
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B-Lymphocytes / cytology*
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B-Lymphocytes / immunology*
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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Cell Differentiation
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Cells, Cultured
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Clone Cells
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / immunology
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Immunoglobulin Idiotypes
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Immunophenotyping
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Transgenic
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Protein-Tyrosine Kinases / metabolism
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Protein-Tyrosine Kinases / physiology*
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Receptors, Antigen, B-Cell / metabolism
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Receptors, Complement 3d / metabolism
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Receptors, IgE / metabolism
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Spleen / cytology
Substances
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Antigens, CD19
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Immunoglobulin Idiotypes
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Receptors, Antigen, B-Cell
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Receptors, Complement 3d
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Receptors, IgE
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Protein-Tyrosine Kinases
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Agammaglobulinaemia Tyrosine Kinase