Internal structure of the chromatin subunit

Nucleic Acids Res. 1974 Nov;1(11):1573-8. doi: 10.1093/nar/1.11.1573.

Abstract

The digestion of chromatin insitu with DNase I reveals, after denaturation, a regular series of single stranded DNA fragments the lengths of which represent multiples of 10 bases. These experiments are compatible with the DNA being on the outside of the chromatin subunit and suggest that the subunit structure itself contains repetitive structural elements. Possible models are discussed.

MeSH terms

  • Animals
  • DNA / chemistry
  • Deoxyribonuclease I / pharmacology
  • Liver / chemistry
  • Liver / ultrastructure
  • Models, Biological
  • Nucleic Acid Denaturation
  • Nucleosomes / drug effects
  • Nucleosomes / ultrastructure*
  • Protein Denaturation
  • Rats

Substances

  • Nucleosomes
  • DNA
  • Deoxyribonuclease I