Abstract
A gene cluster for the non-ribosomal synthesis of a peptide of unknown structure has been identified in the partial genome sequence of Streptomyces coelicolor. Using molecular and computational analyses, the total structure of a tripeptide siderophore synthesized by the non-ribosomal peptide synthetase within the cluster has been deduced from the translated sequence of its encoding gene. This represents a novel method for the structural assignment of natural products from genome sequence data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Computational Biology
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Genome, Bacterial*
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Molecular Sequence Data
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Multigene Family
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Oligopeptides / biosynthesis
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Oligopeptides / chemistry*
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Oligopeptides / genetics
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Peptide Biosynthesis
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Peptide Synthases / chemistry*
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Peptide Synthases / genetics*
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Peptide Synthases / metabolism
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Protein Structure, Tertiary
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Siderophores / biosynthesis
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Siderophores / chemistry*
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Siderophores / genetics
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Streptomyces / enzymology
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Streptomyces / genetics*
Substances
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Oligopeptides
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Siderophores
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coelichelin
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Peptide Synthases
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non-ribosomal peptide synthase