Abstract
The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Animals, Genetically Modified
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Base Sequence
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans Proteins*
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Disorders of Sex Development
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Female
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Genes, Helminth / genetics
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Genetic Complementation Test
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Gonads / cytology
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Gonads / growth & development*
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Gonads / metabolism
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Helminth Proteins / genetics*
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Male
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Phenotype
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Point Mutation
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RNA, Helminth / genetics
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Retinoblastoma Protein / genetics
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Retinoblastoma Protein / metabolism*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Caenorhabditis elegans Proteins
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DNA, Complementary
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Helminth Proteins
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LIN-9 protein, C elegans
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RNA, Helminth
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Retinoblastoma Protein
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lin-36 protein, C elegans
Associated data
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GENBANK/AF269693
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GENBANK/AF269694