Abstract
The relative ability of Th1 and Th2 T cells to help B cells remains controversial as do the mechanisms by which both T cell subsets provide help in vivo. Whether this help affects the clonal expansion and/or differentiation of B cells has been difficult to assess due to the low frequency of Ag-specific T and B lymphocytes. We have employed a novel technique to directly monitor the clonal expansion of Ag-specific T and B lymphocytes in vivo. OVA-specific TCR transgenic T lymphocytes were polarized toward a Th1 or Th2 phenotype in vitro. These cells were then transferred into syngeneic recipients, along with B cell receptor transgenic hen egg lysozyme-specific B lymphocytes. Our results indicate that Th1 and Th2 cells support B cell responses to a similar extent in vivo and that they achieve this in the same manner by migrating into B cell follicles to promote CD154-dependent B cell clonal expansion and Ab production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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Antibodies, Monoclonal / administration & dosage
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Antibody Formation* / genetics
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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B-Lymphocytes / transplantation
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CD40 Antigens / metabolism
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CD40 Ligand
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Movement / genetics
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Cell Movement / immunology
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Cell Polarity / genetics
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Cell Polarity / immunology
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Cells, Cultured
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Clone Cells
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Cytokines / biosynthesis
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology
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Immunophenotyping
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Ligands
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Lymphocyte Activation* / genetics
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Lymphocyte Cooperation* / genetics
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Male
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Membrane Glycoproteins / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Transgenic
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Ovalbumin / immunology
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Th1 Cells / immunology
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Th1 Cells / metabolism
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Th1 Cells / transplantation*
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Th2 Cells / immunology
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Th2 Cells / metabolism
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Th2 Cells / transplantation*
Substances
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Antibodies, Monoclonal
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CD40 Antigens
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Cytokines
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Epitopes, T-Lymphocyte
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Ligands
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Membrane Glycoproteins
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CD40 Ligand
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Ovalbumin