Abstract
We have cloned the full length of a novel cDNA, named ring finger protein 21 (RNF21), composed of the RING finger-B box-coiled coil (RBCC) domain and the B30.2 domain, which are characteristic of the RBCC-B30.2 family. As a structural feature, the RNF21 cDNA possessed at least three kinds of isoforms, due to alternative splicing, consisting of the long form with the RBCC-RBCC-B30.2 domain, the medium form with the RBCC-B30.2 domain, and the short form with only the RBCC domain. Moreover, respective transcripts corresponding to the three isoforms were detected in various human organs by reverse transcription-PCR and Northern blot analyses. Interestingly, the medium form of the RNF21 mRNA expressed most predominantly was dramatically up-regulated within 8-16 h by interferon stimulation of HeLa cells. These findings suggest that RNF21 is a downstream gene that may mediate interferon's biological action.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Blotting, Northern
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COS Cells
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Carrier Proteins / genetics*
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Chromosome Banding
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Chromosome Mapping
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Chromosomes, Human, Pair 11 / genetics
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Female
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Gene Expression Regulation / drug effects
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HeLa Cells
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Humans
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In Situ Hybridization, Fluorescence
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Interferons / pharmacology
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Male
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Molecular Sequence Data
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Protein Isoforms / genetics
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Protein Structure, Tertiary
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Repetitive Sequences, Amino Acid
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
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Zinc Fingers / genetics*
Substances
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Carrier Proteins
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DNA, Complementary
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Protein Isoforms
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RNA, Messenger
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TRIM34 protein, human
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Interferons
Associated data
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GENBANK/AB039902
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GENBANK/AB039903
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GENBANK/AB039904