Abstract
Regulated transcription of the prospero gene in the Drosophila eye provides a model for how gene expression is specifically controlled by signals from receptor tyrosine kinases. We show that prospero is controlled by signals from the EGF receptor DER and the Sevenless receptor. A direct link is established between DER activation of a transcription enhancer in prospero and binding of two transcription factors that are targets of DER signaling. Binding of the cell-specific Lozenge protein is also required for activation, and overlapping Lozenge protein distribution and DER signaling establishes expression in a subset of equivalent cells competent to respond to Sevenless. We show that Sevenless activates prospero independent of the enhancer and involves targeted degradation of Tramtrack, a transcription repressor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence / genetics
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Binding Sites / genetics
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DNA-Binding Proteins*
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Drosophila / cytology
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Drosophila / embryology*
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Drosophila / genetics*
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Drosophila Proteins*
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Enhancer Elements, Genetic / genetics
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ErbB Receptors / genetics
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ErbB Receptors / metabolism
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Eye / cytology
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Eye / embryology*
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Eye / metabolism
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Eye Proteins / genetics
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Eye Proteins / metabolism
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic / genetics
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Protein Kinases*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins / pharmacology
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptor Protein-Tyrosine Kinases / metabolism
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Receptors, Invertebrate Peptide / genetics
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Receptors, Invertebrate Peptide / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Stem Cells / cytology
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Stem Cells / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic / genetics*
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Up-Regulation / genetics
Substances
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AOP protein, Drosophila
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DNA-Binding Proteins
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Drosophila Proteins
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Eye Proteins
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Membrane Glycoproteins
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Nerve Tissue Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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Receptors, Invertebrate Peptide
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Repressor Proteins
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Transcription Factors
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lz protein, Drosophila
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pnt protein, Drosophila
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pros protein, Drosophila
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ttk protein, Drosophila
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Protein Kinases
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Egfr protein, Drosophila
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ErbB Receptors
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Receptor Protein-Tyrosine Kinases
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sev protein, Drosophila
Associated data
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GENBANK/AF190402
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GENBANK/AF190403
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GENBANK/AF190404
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GENBANK/AF190405