Abstract
Nucleosomes impose a block to transcription that can be overcome in vivo by remodeling complexes such as SNF/SWI and histone modification complexes such as SAGA. Mutations in the major core histones relieve transcriptional repression and bypass the requirement for SNF/SWI and SAGA. We have found that the variant histone H2A.Z regulates gene transcription, and deletion of the gene encoding H2A.Z strongly increases the requirement for SNF/SWI and SAGA. This synthetic genetic interaction is seen at the level of single genes and acts downstream of promoter nucleosome reorganization. H2A.Z is preferentially crosslinked in vivo to intergenic DNA at the PH05 and GAL1 loci, and this association changes with transcriptional activation. These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases
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Alleles
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / physiology
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DNA, Fungal / genetics
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DNA, Fungal / metabolism
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DNA, Intergenic / genetics
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DNA, Intergenic / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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DNA-Binding Proteins / physiology
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Fungal Proteins / genetics
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Fungal Proteins / physiology
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Genes, Essential / genetics
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Genes, Fungal / genetics
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Genes, Fungal / physiology
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Histones / chemistry
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Histones / genetics
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Histones / metabolism*
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Hot Temperature
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Macromolecular Substances
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Membrane Transport Proteins / genetics
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Molecular Conformation
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Nuclear Proteins*
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Nucleosomes / chemistry
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Nucleosomes / genetics
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Nucleosomes / metabolism*
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Phenotype
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Phosphate Transport Proteins*
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Promoter Regions, Genetic / genetics
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Protein Binding
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Protein Kinases / genetics
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Protein Kinases / physiology
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Protein Subunits
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Suppression, Genetic / genetics
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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Chromosomal Proteins, Non-Histone
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DNA, Fungal
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DNA, Intergenic
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DNA-Binding Proteins
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Fungal Proteins
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Histones
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Macromolecular Substances
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Membrane Transport Proteins
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Nuclear Proteins
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Nucleosomes
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Phosphate Transport Proteins
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Protein Subunits
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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phosphate permease
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SPT2 protein, S cerevisiae
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Protein Kinases
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Adenosine Triphosphatases
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SNF2 protein, S cerevisiae