Abstract
Traumatic injury to the adult central nervous system initiates a cascade of cellular and trophic events, culminating in the formation of a reactive gliotic scar through which transected axons fail to regenerate. Levels of fibroblast growth factor-2 (FGF-2), a potent gliogenic and neurotrophic factor, together with its full-length receptor, FGF receptor 1 (FGFR1) are coordinately and significantly increased postinjury in both nuclear and cytoplasmic fractions of extracted cerebral cortex biopsies after a penetrant injury. FGFR1 is colocalized with FGF-2 in the nuclei of reactive astrocytes, and here FGF-2 is associated with nuclear euchromatin. This study unequivocally demonstrates coordinate up-regulation and trafficking of FGF-2 and full-length FGFR1 to the nucleus of reactive astrocytes in an in vivo model of brain injury, thereby implicating a role in nuclear activity for these molecules. However, the precise contribution of nuclear FGF-2/FGFR1 to the pathophysiological response of astrocytes after injury is undetermined.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Active Transport, Cell Nucleus / physiology*
-
Animals
-
Astrocytes / metabolism*
-
Astrocytes / pathology
-
Blotting, Western
-
Brain / metabolism
-
Brain / pathology
-
Cell Nucleus / metabolism
-
Cell Nucleus / ultrastructure
-
Disease Models, Animal
-
Euchromatin / metabolism
-
Euchromatin / ultrastructure
-
Female
-
Fibroblast Growth Factor 2 / metabolism*
-
Gliosis / etiology
-
Gliosis / metabolism
-
Gliosis / pathology
-
Head Injuries, Penetrating / complications
-
Head Injuries, Penetrating / metabolism*
-
Head Injuries, Penetrating / pathology
-
Immunohistochemistry
-
Protein Isoforms / metabolism
-
Rats
-
Rats, Wistar
-
Receptor Protein-Tyrosine Kinases / metabolism*
-
Receptor, Fibroblast Growth Factor, Type 1
-
Receptors, Fibroblast Growth Factor / metabolism*
-
Subcellular Fractions / metabolism
-
Up-Regulation
Substances
-
Euchromatin
-
Protein Isoforms
-
Receptors, Fibroblast Growth Factor
-
Fibroblast Growth Factor 2
-
Fgfr1 protein, rat
-
Receptor Protein-Tyrosine Kinases
-
Receptor, Fibroblast Growth Factor, Type 1