Abstract
We cloned a novel murine gene, designated Hemogen (hemopoietic gene), which was sequentially expressed in active hematopoietic sites and downregulated in the process of blood cell differentiation. Hemogen transcripts were specifically detected in blood islands, primitive blood cells and fetal liver during embryogenesis, and then remained in bone marrow and spleen in adult mice. Immunostaining demonstrated that Hemogen was a nuclear protein. We also identified a human homologue of Hemogen, named EDAG, which was mapped to chromosome 9q22, a leukemia breakpoint. Like Hemogen, EDAG exhibited specific expression in hematopoietic tissues and cells. Taken together, these data are consistent with Hemogen and EDAG playing an important role in hematopoietic development and neoplasms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Bone Marrow / metabolism
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COS Cells
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Cell Nucleus / metabolism*
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Chromosomes, Human, Pair 9*
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Cloning, Molecular
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Down-Regulation
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Hematologic Neoplasms / genetics*
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Hematologic Neoplasms / metabolism*
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Hematopoietic Stem Cells / metabolism*
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Humans
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In Situ Hybridization
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Liver / embryology
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Mice
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Molecular Sequence Data
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Nuclear Proteins / biosynthesis*
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Nuclear Proteins / genetics*
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Plasmids
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Spleen / metabolism
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Tissue Distribution
Substances
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HEMGN protein, human
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Hemgn protein, mouse
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Nuclear Proteins
Associated data
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GENBANK/AF269248
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GENBANK/AF322875