Abstract
The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate developmental signalling specificity. We describe a new gene, fringe connection (frc), that encodes a nucleotide-sugar transporter that transfers UDP-glucuronic acid, UDP-N-acetylglucosamine and possibly UDP-xylose from the cytoplasm into the lumen of the endoplasmic reticulum/Golgi. Embryos with the frc mutation display defects in Wingless, Hedgehog and fibroblast growth factor signalling. Clonal analysis shows that fringe-dependent Notch signalling is disrupted in frc mutant tissue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cytoplasm / metabolism
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Drosophila Proteins
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Endoplasmic Reticulum / metabolism
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Glycosyltransferases / genetics
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Glycosyltransferases / metabolism*
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Golgi Apparatus / metabolism
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Heparitin Sulfate / metabolism*
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Humans
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Molecular Sequence Data
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Morphogenesis
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N-Acetylglucosaminyltransferases*
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Phenotype
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Sequence Alignment
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Sequence Homology, Amino Acid
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Signal Transduction / physiology*
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Uridine Diphosphate Glucuronic Acid / metabolism
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Uridine Diphosphate N-Acetylglucosamine / metabolism
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Uridine Diphosphate Xylose / metabolism
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Wings, Animal / embryology
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Wings, Animal / growth & development
Substances
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Drosophila Proteins
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Uridine Diphosphate Glucuronic Acid
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Uridine Diphosphate Xylose
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Uridine Diphosphate N-Acetylglucosamine
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Heparitin Sulfate
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Glycosyltransferases
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N-Acetylglucosaminyltransferases
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fng protein, Drosophila