Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray

Yosuke Mizuno; Yusuke Sotomaru; Yukiko Katsuzawa; Tomohiro Kono; Makiko Meguro; Mitsuo Oshimura; Jun Kawai; Yasuhiro Tomaru; Hidenori Kiyosawa; Itoshi Nikaido; Hiroshi Amanuma; Yoshihide Hayashizaki; Yasushi Okazaki

doi:10.1006/bbrc.2002.6370

Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray

Biochem Biophys Res Commun. 2002 Feb 8;290(5):1499-505. doi: 10.1006/bbrc.2002.6370.

Authors

Yosuke Mizuno¹, Yusuke Sotomaru, Yukiko Katsuzawa, Tomohiro Kono, Makiko Meguro, Mitsuo Oshimura, Jun Kawai, Yasuhiro Tomaru, Hidenori Kiyosawa, Itoshi Nikaido, Hiroshi Amanuma, Yoshihide Hayashizaki, Yasushi Okazaki

Affiliation

¹ Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230-0045, Japan.

PMID: 11820791
DOI: 10.1006/bbrc.2002.6370

Abstract

Genes differentially expressed between parthenogenetic and androgenetic embryos are candidates for the identification of imprinted genes, which are expressed specifically from the maternal or paternal allele. To search for genes differentially expressed between parthenogenetic and androgenetic embryos, we used the RIKEN full-length enriched mouse cDNA microarray. The 25 candidates obtained included 8 known imprinted genes (such as IgfII, Snrpn, and Neuronatin) and 3 new ones--Asb4 (ankyrin repeat and SOCS box-containing protein 4), Ata3 (amino acid transport system A3), and Decorin--which were confirmed by using normal diploid embryos from the reciprocal F1 crosses of B6 and JF1 mice. The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Amino Acid Transport System A / biosynthesis
Amino Acid Transport System A / genetics*
Animals
Ankyrin Repeat / genetics*
Autoantigens / biosynthesis
Autoantigens / genetics
Carrier Proteins / biosynthesis
Carrier Proteins / genetics*
Decorin
Embryo, Mammalian
Extracellular Matrix Proteins
Female
Gene Expression Profiling
Genetic Testing / methods*
Genomic Imprinting*
Insulin-Like Growth Factor II / biosynthesis
Insulin-Like Growth Factor II / genetics
Male
Membrane Proteins / biosynthesis
Membrane Proteins / genetics
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / biosynthesis
Nerve Tissue Proteins / genetics
Oligonucleotide Array Sequence Analysis / methods*
Proteoglycans / biosynthesis
Proteoglycans / genetics
Ribonucleoproteins, Small Nuclear*
snRNP Core Proteins

Substances

Amino Acid Transport System A
Autoantigens
Carrier Proteins
Dcn protein, mouse
Dcn protein, rat
Decorin
Extracellular Matrix Proteins
Membrane Proteins
Nerve Tissue Proteins
Nnat protein, mouse
Proteoglycans
Ribonucleoproteins, Small Nuclear
Slc38a4 protein, mouse
Slc38a4 protein, rat
snRNP Core Proteins
Insulin-Like Growth Factor II