Abstract
Arabidopsis thaliana AtBI-1 is an orthologue of mammalian Bax inhibitor-1 capable of suppressing Bax-induced cell death in yeast as well as mammalian cells. Here we investigated whether or not AtBI-1 suppresses Bax-induced cell death using human fibrosarcoma HT1080 cells. Surprisingly, AtBI-1 did not block Bax-induced cell death, but it triggered apoptotic cell death in mammalian cells. The proapoptotic effect of AtBI-1 was blocked by the X-linked caspase inhibitor XIAP, suggesting that the cell death caused by AtBI-1 is similar to that caused by Bax.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Apoptosis Regulatory Proteins
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Apoptosis*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / pharmacology*
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Caspase Inhibitors
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / pharmacology*
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Models, Biological
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Proteins / pharmacology
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Proto-Oncogene Proteins / antagonists & inhibitors*
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Proto-Oncogene Proteins c-bcl-2 / antagonists & inhibitors*
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Recombinant Proteins / pharmacology
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Species Specificity
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Tumor Cells, Cultured
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X-Linked Inhibitor of Apoptosis Protein
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bcl-2-Associated X Protein
Substances
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ATBI-1 protein, Arabidopsis
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Apoptosis Regulatory Proteins
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Arabidopsis Proteins
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BAX protein, human
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Caspase Inhibitors
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Membrane Proteins
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Recombinant Proteins
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TMBIM6 protein, human
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X-Linked Inhibitor of Apoptosis Protein
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XIAP protein, human
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bcl-2-Associated X Protein