Abstract
Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin-bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carrier Proteins / metabolism
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Cell Cycle
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Cycle Proteins / physiology
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Chromatin / metabolism
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DNA / metabolism
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DNA Replication* / physiology
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DNA-Binding Proteins / metabolism*
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Egg Proteins / metabolism
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Expressed Sequence Tags
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Kinetics
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Macromolecular Substances
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Minichromosome Maintenance Proteins
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Nuclear Proteins / metabolism
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Oocytes / metabolism
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Protein Binding
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Protein Serine-Threonine Kinases / physiology
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Replication Origin / genetics*
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Saccharomyces cerevisiae Proteins*
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Xenopus Proteins / metabolism*
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Xenopus laevis / metabolism*
Substances
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CDC45 protein, S cerevisiae
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Carrier Proteins
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Cell Cycle Proteins
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Chromatin
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DNA-Binding Proteins
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Egg Proteins
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Macromolecular Substances
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Mcm10 protein, Xenopus
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Nuclear Proteins
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Saccharomyces cerevisiae Proteins
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Xenopus Proteins
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DNA
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CDC7 protein, S cerevisiae
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CDC7 protein, Xenopus
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Protein Serine-Threonine Kinases
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Minichromosome Maintenance Proteins