High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks

Appl Opt. 2002 Aug 1;41(22):4704-8. doi: 10.1364/ao.41.004704.

Abstract

We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms. We expect that it will be used for measuring biological and industrial active samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Equipment Design
  • Heart Ventricles
  • Lenses*
  • Microscopy, Confocal / instrumentation*
  • Myocardium / cytology
  • Myocardium / metabolism
  • Rats

Substances

  • Calcium