Abstract
Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tlc, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tlc. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Body Patterning / genetics*
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins / genetics
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Bone Morphogenetic Proteins / metabolism
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Cell Differentiation / genetics
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Cell Lineage / genetics
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Cytoskeletal Proteins
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Denervation
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Gastrula / cytology
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Gastrula / metabolism*
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Gene Expression Regulation / physiology*
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Immunohistochemistry
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Intracellular Signaling Peptides and Proteins
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Mesencephalon / cytology
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Mesencephalon / embryology
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Mesencephalon / metabolism
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Molecular Sequence Data
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Mutation / genetics
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Neurons / cytology
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Neurons / metabolism
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Phylogeny
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Prosencephalon / cytology
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Prosencephalon / embryology
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Prosencephalon / metabolism
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Proteins / genetics
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Proteins / isolation & purification*
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Proteins / metabolism
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Signal Transduction / genetics
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Telencephalon / cytology
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Telencephalon / embryology*
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Telencephalon / metabolism
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Transforming Growth Factor beta*
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Wnt Proteins
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Zebrafish / embryology*
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Zebrafish / genetics
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Zebrafish / metabolism
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Zebrafish Proteins / genetics
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Zebrafish Proteins / isolation & purification*
Substances
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins
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Cytoskeletal Proteins
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Intracellular Signaling Peptides and Proteins
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Proteins
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Proto-Oncogene Proteins
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Transforming Growth Factor beta
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WD repeat containing planar cell polarity effector
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Wnt Proteins
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Zebrafish Proteins
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bmp7a protein, zebrafish
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wnt8a protein, zebrafish
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wnt8b protein, zebrafish