Abstract
Delamination of premigratory neural crest cells from the dorsal neural tube depends both upon environmental signals and cell-intrinsic mechanisms and is a prerequisite for cells to engage in migration. Here we show that avian neural crest cells synchronously emigrate from the neural tube in the S phase of the cell cycle. Furthermore, specific inhibition of the transition from G1 to S both in ovo and in explants blocks delamination, whereas arrest at the S or G2 phases has no immediate effect. Thus, the events taking place during G1 that control the transition from G1 to S are necessary for the epithelial to mesenchymal conversion of crest precursors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antimetabolites / pharmacology
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Bromodeoxyuridine / pharmacology
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Cell Cycle / physiology
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Cell Differentiation
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Cell Movement
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Cell Nucleus / physiology
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Chick Embryo
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Culture Techniques
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DNA / biosynthesis
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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G1 Phase / physiology
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Kinetin
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Mimosine / pharmacology
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Models, Biological
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Morphogenesis
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MyoD Protein / chemistry
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MyoD Protein / metabolism
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Neural Crest / cytology
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Neural Crest / embryology*
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Neural Crest / physiology
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Purines / pharmacology
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Quail / embryology
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S Phase / physiology
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Transcription Factors / metabolism
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Tyrphostins / pharmacology
Substances
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Antimetabolites
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Enzyme Inhibitors
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MyoD Protein
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Purines
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Transcription Factors
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Tyrphostins
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alpha-cyano-(3,4-dihydroxy)-N-(3-phenylpropyl)cinnamide
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Mimosine
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olomoucine
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DNA
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Bromodeoxyuridine
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Kinetin