Excess dNTPs minimize RNA hydrolysis during reverse transcription

Biotechniques. 2002 Nov;33(5):984, 986, 988 passim. doi: 10.2144/02335bm03.
No abstract available

MeSH terms

  • Avian Sarcoma Viruses / enzymology
  • DNA, Complementary / biosynthesis*
  • Deoxyadenine Nucleotides / pharmacology*
  • Deoxycytosine Nucleotides / pharmacology*
  • Deoxyguanine Nucleotides / pharmacology*
  • Drug Stability
  • Electrophoresis, Agar Gel
  • Half-Life
  • Hot Temperature
  • Hydrolysis / drug effects
  • Magnesium / pharmacology
  • Moloney murine leukemia virus / enzymology
  • Nucleic Acid Denaturation
  • RNA / metabolism*
  • RNA-Directed DNA Polymerase / metabolism*
  • Thymine Nucleotides / pharmacology*
  • Transcription, Genetic*
  • Viral Proteins / metabolism*

Substances

  • DNA, Complementary
  • Deoxyadenine Nucleotides
  • Deoxycytosine Nucleotides
  • Deoxyguanine Nucleotides
  • Thymine Nucleotides
  • Viral Proteins
  • 2'-deoxycytidine 5'-triphosphate
  • RNA
  • deoxyguanosine triphosphate
  • RNA-Directed DNA Polymerase
  • Magnesium
  • 2'-deoxyadenosine triphosphate
  • thymidine 5'-triphosphate