Abstract
In inbred mouse strains, permissiveness to intracellular replication of Legionella pneumophila is controlled by a single locus (Lgn1), which maps to a region within distal Chromosome 13 that contains multiple copies of the gene baculoviral IAP repeat-containing 1 (Birc1, also called Naip; refs. 1-3). Genomic BAC clones from the critical interval were transferred into transgenic mice to functionally complement the Lgn1-associated susceptibility of A/J mice to L. pneumophila. Here we report that two independent BAC clones that rescue susceptibility have an overlapping region of 56 kb in which the entire Lgn1 transcript must lie. The only known full-length transcript coded in this region is Birc1e (also called Naip5).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chromosomes, Mammalian / genetics
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Female
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Genetic Predisposition to Disease / genetics*
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Legionella pneumophila / physiology*
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Legionnaires' Disease / genetics*
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Legionnaires' Disease / microbiology*
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Macrophages, Peritoneal / immunology
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Macrophages, Peritoneal / microbiology
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Male
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Mice
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Mice, Inbred Strains
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Mice, Transgenic
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Neuronal Apoptosis-Inhibitory Protein
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Physical Chromosome Mapping
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
Substances
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Naip1 protein, mouse
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Naip5 protein, mouse
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Nerve Tissue Proteins
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Neuronal Apoptosis-Inhibitory Protein
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RNA, Messenger
Associated data
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GENBANK/AF131205
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GENBANK/AF242431
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RefSeq/NM_010870