Abstract
A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Anticodon
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Codon*
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Escherichia coli / genetics*
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Genetic Code*
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Mice
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Phenylalanine / genetics
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Plasmids / genetics
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Promoter Regions, Genetic
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RNA, Transfer, Phe / genetics*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tetrahydrofolate Dehydrogenase / genetics*
Substances
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Anticodon
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Codon
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RNA, Transfer, Phe
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Phenylalanine
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Tetrahydrofolate Dehydrogenase