Abstract
We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacteria, Anaerobic / ultrastructure*
-
Biofilms / growth & development*
-
Bioreactors
-
Desiccation*
-
Methanosarcinales / ultrastructure
-
Microscopy, Electron, Scanning / methods*
-
Organosilicon Compounds*
-
Sewage / microbiology*
-
Tissue Fixation
Substances
-
Organosilicon Compounds
-
Sewage
-
hexamethylsilazane