Mesothoracic ventral unpaired median (mesVUM) neurons in the blowfly Calliphora erythrocephala

The study describes five ventral unpaired median neurons in the mesothoracic neuromere of the fused thoracic ganglion of Calliphora identified by biocytin staining (mesVUM neurons). The group comprises four efferent neurons and one interneuron which are characterized by a common soma cluster in the ventral midline of the neuromere, bifurcating primary neurites and bilaterally symmetrical arborizations. Respective soma clusters of not-yet-identified VUM neurons were also found in the prothoracic, metathoracic, and abdominal neuromeres. The efferent mesVUM neurons are associated with the flight system. Their main arborizations are located in the mesothoracic wing neuropil and their bilateral axons terminate at the flight control muscles, the flight starter muscles, the flight power muscles, or at myocuticular junctions of the latter. In contrast, an association of the interneuron with a particular functional system is not apparent. The arborizations of the neuron are intersegmental and invade all thoracic neuromeres. A further difference between the two types of neurons regards their somatic action potentials, which are overshooting in the efferent neurons and strongly attenuated in the interneuron. Immunocytochemical stainings revealed four clusters of octopamine-immunoreactive (OA-IR) somata in the thoracic ganglion, which reside in the same positions as the VUM somata. We regard this as strong evidence that all groups of VUM neurons contain OA-IR cells and that, in particular, the identified efferent mesVUM neurons are OA-IR. Our results demonstrate that the mesVUM neurons of Calliphora have similar morphological, electrophysiological, and presumably also immunocytochemical characteristics as the unpaired median neurons of other insects.