Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II

Antonin Morillon; Nickoletta Karabetsou; Justin O'Sullivan; Nicholas Kent; Nicholas Proudfoot; Jane Mellor

doi:10.1016/s0092-8674(03)00880-8

Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II

Cell. 2003 Nov 14;115(4):425-35. doi: 10.1016/s0092-8674(03)00880-8.

Authors

Antonin Morillon¹, Nickoletta Karabetsou, Justin O'Sullivan, Nicholas Kent, Nicholas Proudfoot, Jane Mellor

Affiliation

¹ Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.

PMID: 14622597
DOI: 10.1016/s0092-8674(03)00880-8

Abstract

We demonstrate that distinct forms of the yeast chromatin-remodeling enzyme Isw1p sequentially regulate each stage of the transcription cycle. The Isw1a complex (Iswlp/Ioc3p) represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome, whereas the Isw1b complex (Iswlp/Ioc2p/Ioc4p) acts within coding regions to control the amount of RNA polymerase (RNAPII) released into productive elongation and to coordinate elongation with termination and pre-mRNA processing. These effects of Isw1b are controlled via phosphorylation of the heptad repeat carboxy-terminal domain (CTD) of RNAPII and methylation of the chromatin template. The transcription elongation factor Spt4p antagonizes Isw1p and overcomes the Isw1p dependent pausing of RNAPII at the onset of the elongation cycle. Overall these studies establish the central role played by Isw1p in the coordination of transcription.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism*
Chromatin Assembly and Disassembly*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Gene Expression Regulation, Fungal*
Gene Silencing
Genes, Fungal / genetics
Histones / metabolism
Methylation
Nuclear Proteins / metabolism
Nucleosomes / metabolism
Phosphorylation
Phosphoserine / metabolism
Protein Conformation
RNA Polymerase II / chemistry
RNA Polymerase II / metabolism*
Repressor Proteins / genetics
Repressor Proteins / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism
Transcription, Genetic*
Transcriptional Elongation Factors / metabolism

Substances

DNA-Binding Proteins
DST1 protein, S cerevisiae
Histones
Nuclear Proteins
Nucleosomes
Repressor Proteins
SPT4 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Transcriptional Elongation Factors
Phosphoserine
RNA Polymerase II
Adenosine Triphosphatases
ISW1 protein, S cerevisiae