Some precautions in using chelators to buffer metals in biological solutions

Chris Patton; Stuart Thompson; David Epel

doi:10.1016/j.ceca.2003.10.006

Some precautions in using chelators to buffer metals in biological solutions

Cell Calcium. 2004 May;35(5):427-31. doi: 10.1016/j.ceca.2003.10.006.

Authors

Chris Patton¹, Stuart Thompson, David Epel

Affiliation

¹ Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950, USA. cpatton@stanford.edu

PMID: 15003852
DOI: 10.1016/j.ceca.2003.10.006

Abstract

Chelators and associated computer programs are commonly used to buffer metal ions in biological experiments. This communication discusses common misunderstandings and pitfalls in use of these buffers and provides information on choosing the best metal buffer for different experimental situations.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Buffers
Chelating Agents / chemistry*
Hydrogen-Ion Concentration
Metals / chemistry*
Models, Chemical*
Solutions / chemistry

Substances

Buffers
Chelating Agents
Metals
Solutions

Abstract

Publication types

MeSH terms

Substances

Grants and funding