Abstract
The GTPase Arl3p is required to recruit a second GTPase, Arl1p, to the Golgi in Saccharomyces cerevisiae. Arl1p binds to the GRIP domain, which is present in a number of long coiled-coil proteins or 'golgins'. Here we show that Arl3p is not myristoylated like most members of the Arf family, but is instead amino-terminally acetylated by the NatC complex. Targeting of Arl3p also requires a Golgi membrane protein Sys1p. The human homologues of Arl3p (Arf-related protein 1 (ARFRP1)) and Sys1p (hSys1) can be isolated in a complex after chemical cross-linking. This suggests that the targeting of ARFRP1/Arl3p to the Golgi is mediated by a direct interaction between its acetylated N terminus and Sys1p/hSys1.
MeSH terms
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ADP-Ribosylation Factor 1 / genetics
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ADP-Ribosylation Factor 1 / metabolism
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ADP-Ribosylation Factors / genetics
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ADP-Ribosylation Factors / metabolism*
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Acetylation
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Acetyltransferases / metabolism
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Amino Acid Sequence
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Animals
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Arylamine N-Acetyltransferase / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Endoplasmic Reticulum / metabolism
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Golgi Apparatus / metabolism*
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Humans
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Macromolecular Substances
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Monomeric GTP-Binding Proteins*
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Protein Structure, Tertiary
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Protein Transport / physiology
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Sequence Alignment
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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IMH1 protein, S cerevisiae
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Macromolecular Substances
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Membrane Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Sys1 protein, S cerevisiae
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Vesicular Transport Proteins
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Acetyltransferases
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MAK3 protein, S cerevisiae
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Arylamine N-Acetyltransferase
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ADP-ribosylation factor related proteins
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ARL1 protein, S cerevisiae
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ADP-Ribosylation Factor 1
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ADP-Ribosylation Factors
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ARL3 protein, S cerevisiae
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ARL3 protein, human
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Monomeric GTP-Binding Proteins