Abstract
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
MeSH terms
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Adolescent
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Adult
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Antibodies, Viral / isolation & purification
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Child
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Child, Preschool
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Communicable Diseases, Emerging / diagnosis*
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Communicable Diseases, Emerging / immunology
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Communicable Diseases, Emerging / physiopathology
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Female
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Fluorescent Antibody Technique, Indirect / methods*
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Hong Kong
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Humans
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Male
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Middle Aged
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Reverse Transcriptase Polymerase Chain Reaction
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Severe Acute Respiratory Syndrome / diagnosis*
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Severe Acute Respiratory Syndrome / immunology
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Severe Acute Respiratory Syndrome / physiopathology