Abstract
The centrosome plays diverse roles throughout the cellular mitotic cycle and in post-mitotic cells. Analysis of centrosome position and dynamics in living murine cells has been limited due to a lack of adequate reporters and currently requires either cell fixation/immunostaining or transfection with centrosome reporters. Here we describe the generation and characterization of a transgenic mouse line that constitutively expresses green fluorescent protein-labeled Centrin-2 (GFP-CETN2). The phenotype of the mouse is indistinguishable from wild-type and it displays a single pair of fluorescent centrioles in cells of every organ and time point examined. This model will be helpful for visualizing the centrosome in multiple experimental conditions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Calcium-Binding Proteins / analysis
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Calcium-Binding Proteins / genetics*
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Calcium-Binding Proteins / metabolism
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Cell Nucleus / ultrastructure
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Centrioles / ultrastructure
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Centrosome / metabolism
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Centrosome / ultrastructure*
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Embryo, Mammalian / cytology
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Fibroblasts / ultrastructure
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Green Fluorescent Proteins / analysis
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Green Fluorescent Proteins / genetics*
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Mice
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Mice, Transgenic / genetics*
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Microscopy, Fluorescence
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Recombinant Fusion Proteins / analysis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
Substances
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Calcium-Binding Proteins
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Cetn2 protein, mouse
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Recombinant Fusion Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins