Abstract
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Culture Techniques / methods*
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Cell Nucleus / genetics*
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Cells, Cultured
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Culture Media / pharmacology
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DNA, Plant / genetics*
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Molecular Sequence Data
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Multienzyme Complexes / deficiency
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Multienzyme Complexes / genetics
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Mutation / genetics
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Orotate Phosphoribosyltransferase / deficiency
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Orotate Phosphoribosyltransferase / genetics
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Orotic Acid / analogs & derivatives*
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Orotic Acid / pharmacology
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Orotidine-5'-Phosphate Decarboxylase / deficiency
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Orotidine-5'-Phosphate Decarboxylase / genetics
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Recombination, Genetic / genetics*
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Rhodophyta / genetics*
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Rhodophyta / metabolism*
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Transformation, Genetic / genetics*
Substances
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Culture Media
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DNA, Plant
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Multienzyme Complexes
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Orotic Acid
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uridine 5'-monophosphate synthase
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5-fluoroorotic acid
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Orotate Phosphoribosyltransferase
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Orotidine-5'-Phosphate Decarboxylase