Abstract
Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriocins / classification
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Bacteriocins / pharmacology*
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Base Sequence
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Chromosome Mapping
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial / genetics
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Gene Expression / drug effects
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Genes, Bacterial
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Lactococcus lactis / drug effects*
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Lactococcus lactis / enzymology*
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Lactococcus lactis / genetics
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Listeria monocytogenes / enzymology*
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Listeria monocytogenes / genetics*
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Nisin / pharmacology
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Operon
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Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
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Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
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Plasmids / genetics
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Protein Subunits
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Bacteriocins
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DNA, Bacterial
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Protein Subunits
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Recombinant Proteins
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Nisin
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-mannose phosphotransferase