Abstract
Centrins are calmodulin-like proteins that function in the duplication of microtubule-organizing centres. Here we describe a new function of the yeast centrin Cdc31. We show that overproduction of a sequence, termed CID, in the carboxy-terminal domain of the nuclear export factor Sac3 titrates Cdc31, causing a dominant-lethal phenotype and a block in spindle pole body (SPB) duplication. Under normal conditions, the CID motif recruits Cdc31 and Sus1 (a subunit of the SAGA transcription complex) to the Sac3-Thp1 complex, which functions in mRNA export together with specific nucleoporins at the nuclear basket. A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus*
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Calcium-Binding Proteins / metabolism
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Calcium-Binding Proteins / physiology*
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Cell Cycle Proteins / metabolism
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Cell Cycle Proteins / physiology*
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Fungal Proteins / metabolism
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Fungal Proteins / physiology
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Nuclear Proteins / metabolism
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Nucleocytoplasmic Transport Proteins
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Porins
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Protein Binding
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / metabolism
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae Proteins / metabolism
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Spindle Apparatus / metabolism
Substances
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CDC31 protein, S cerevisiae
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Calcium-Binding Proteins
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Cell Cycle Proteins
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Fungal Proteins
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Nuclear Proteins
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Nucleocytoplasmic Transport Proteins
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Porins
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RNA, Messenger
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RNA-Binding Proteins
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Ribonucleoproteins
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SAC3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Sus1 protein, S cerevisiae
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Thp1 protein, S cerevisiae