The ABC's (and XYZ's) of peptide sequencing

Hanno Steen; Matthias Mann

doi:10.1038/nrm1468

The ABC's (and XYZ's) of peptide sequencing

Nat Rev Mol Cell Biol. 2004 Sep;5(9):699-711. doi: 10.1038/nrm1468.

Authors

Hanno Steen¹, Matthias Mann

Affiliation

¹ Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.

PMID: 15340378
DOI: 10.1038/nrm1468

Abstract

Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key - but little understood - technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amino Acid Sequence
Computational Biology
Databases, Factual
Mass Spectrometry / instrumentation
Mass Spectrometry / methods
Molecular Structure
Peptides* / analysis
Peptides* / genetics
Protein Isoforms / analysis
Protein Isoforms / genetics
Proteomics / methods*
Sequence Analysis, Protein / instrumentation
Sequence Analysis, Protein / methods*

Substances

Peptides
Protein Isoforms