A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra

J Magn Reson. 2005 Feb;172(2):324-8. doi: 10.1016/j.jmr.2004.11.005.

Abstract

Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium-Binding Proteins / chemistry*
  • Carbon Isotopes
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*

Substances

  • Calcium-Binding Proteins
  • Carbon Isotopes
  • Nitrogen Isotopes
  • oncomodulin