An alternative method for Plasmodium culture synchronization

Exp Parasitol. 2005 Mar;109(3):195-7. doi: 10.1016/j.exppara.2004.11.012.

Abstract

Since the synchronization of Plasmodium falciparum has become an essential tool in research, we have investigated the use of a commercial gelatine solution, Plasmion, to replace Plasmagel, which is now difficult to obtain. This method also avoids the use of techniques based on Percoll-glucose gradients. The Plasmion-based technique proved to be a good method and could become an alternative to Plasmagel.

MeSH terms

  • Animals
  • Cells, Cultured
  • Erythrocytes / parasitology*
  • Gelatin / analogs & derivatives*
  • Humans
  • Plasma Substitutes
  • Plasmodium falciparum / physiology*

Substances

  • Plasma Substitutes
  • plasmagel
  • plasmion
  • Gelatin