Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis

Biotechnol Lett. 2005 Feb;27(4):259-63. doi: 10.1007/s10529-004-8295-1.

Abstract

To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the beta-glucosidase gene from Ruminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose-fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the beta-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellobiose / metabolism*
  • Cellulose / metabolism
  • Ethanol / metabolism*
  • Genetic Enhancement / methods*
  • Protein Engineering / methods*
  • Recombinant Fusion Proteins / metabolism
  • Ruminococcus / genetics
  • Ruminococcus / metabolism*
  • Zymomonas / genetics
  • Zymomonas / metabolism*
  • beta-Glucosidase / genetics*
  • beta-Glucosidase / metabolism*

Substances

  • Recombinant Fusion Proteins
  • Cellobiose
  • Ethanol
  • Cellulose
  • beta-Glucosidase