Abstract
A class of highly fluorescent and photostable core-shell nanoparticles from a modified Stober synthesis in the size range of 20-30 nm is described. These nanoparticles are monodisperse in solution, 20 times brighter, and more photostable than their constituent fluorophore, and are amenable to specific labeling of biological macromolecules for bioimaging experiments. The photophysical characteristics of the encapsulated fluorophores differ from their solution properties. This raises the possibility of tuning nanoparticle structure toward enhanced radiative properties, making them an attractive material platform for a diverse range of applications.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Fluorescent Dyes / chemistry*
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Immunoglobulin E / metabolism
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Mast Cells / cytology
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Mast Cells / metabolism
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Microscopy, Electron, Transmission
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Nanostructures / chemistry*
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Nanostructures / ultrastructure
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Photobleaching*
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Rats
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Receptors, IgE / metabolism
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Rhodamines / chemistry
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Silicon Dioxide / chemistry*
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Spectrometry, Fluorescence
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Tumor Cells, Cultured
Substances
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Fluorescent Dyes
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Receptors, IgE
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Rhodamines
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Immunoglobulin E
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tetramethylrhodamine isothiocyanate
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Silicon Dioxide