Abstract
We have identified a particularly clear case of compensatory transcriptional regulation among ER chaperones in Caenorhabditis elegans using a GFP reporter transgene that is under the control of the promoter of hsp-4, a C. elegans homolog of GRP-78/BiP. Knockdown by RNA interference of 9 known or predicted ER chaperones induced hsp-4 upregulation via the ire-1/xbp-1 signaling cascade employed in the unfolded protein response. We show that this compensatory regulation is specific for ER chaperones, not dependent on RNA interference, and required for maintaining viability in worms containing a deletion of the hsp-3 gene.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / cytology*
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Endoplasmic Reticulum / metabolism*
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Gene Expression Regulation / genetics*
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Genes, Reporter / genetics
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Heat-Shock Proteins / genetics
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism*
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Mutation / genetics
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RNA Interference
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Regulatory Factor X Transcription Factors
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic / genetics
Substances
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Caenorhabditis elegans Proteins
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DNA-Binding Proteins
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Heat-Shock Proteins
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Hsp-3 protein, C elegans
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Hsp-4 protein, C elegans
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Molecular Chaperones
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Regulatory Factor X Transcription Factors
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Transcription Factors