Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation

Mihaela Diaconu; Ute Kothe; Frank Schlünzen; Niels Fischer; Jörg M Harms; Alexander G Tonevitsky; Holger Stark; Marina V Rodnina; Markus C Wahl

doi:10.1016/j.cell.2005.04.015

Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation

Cell. 2005 Jul 1;121(7):991-1004. doi: 10.1016/j.cell.2005.04.015.

Authors

Mihaela Diaconu¹, Ute Kothe, Frank Schlünzen, Niels Fischer, Jörg M Harms, Alexander G Tonevitsky, Holger Stark, Marina V Rodnina, Markus C Wahl

Affiliation

¹ Röntgenkristallographie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, D-37077 Göttingen, Germany.

PMID: 15989950
DOI: 10.1016/j.cell.2005.04.015

Abstract

The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12 N-terminal-domain dimers, refine the structure of an archaeal L10E N-terminal domain on the 50S subunit, and identify these elements in cryo-electron-microscopic reconstructions of Escherichia coli ribosomes. The mobile C-terminal helix alpha8 of L10 carries three L7/12 dimers in T. maritima and two in E. coli, in concordance with the different length of helix alpha8 of L10 in these organisms. The stalk is organized into three elements (stalk base, L10 helix alpha8-L7/12 N-terminal-domain complex, and L7/12 C-terminal domains) linked by flexible connections. Highly mobile L7/12 C-terminal domains promote recruitment of translation factors to the ribosome and stimulate GTP hydrolysis by the ribosome bound factors through stabilization of their active GTPase conformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Binding Sites / physiology
Cryoelectron Microscopy
Crystallography, X-Ray
Enzyme Activation / physiology
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli / ultrastructure
GTP Phosphohydrolases / metabolism*
Models, Molecular
Molecular Sequence Data
Prokaryotic Initiation Factors / metabolism
Protein Structure, Secondary / physiology
Protein Structure, Tertiary / physiology
Protein Subunits / chemistry
Protein Subunits / metabolism
RNA, Ribosomal / metabolism
Ribosomal Protein L10
Ribosomal Proteins / chemistry*
Ribosomal Proteins / metabolism*
Ribosomal Proteins / ultrastructure
Ribosomes / genetics
Ribosomes / metabolism*
Ribosomes / ultrastructure
Thermotoga maritima / genetics
Thermotoga maritima / metabolism*
Thermotoga maritima / ultrastructure

Substances

Prokaryotic Initiation Factors
Protein Subunits
RNA, Ribosomal
Ribosomal Proteins
ribosomal protein L7-L12
GTP Phosphohydrolases

Associated data

PDB/1ZAV
PDB/1ZAW
PDB/1ZAX
PDB/1ZB4