In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope

Benjamin A Flusberg; Juergen C Jung; Eric D Cocker; Erik P Anderson; Mark J Schnitzer

doi:10.1364/ol.30.002272

In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope

Opt Lett. 2005 Sep 1;30(17):2272-4. doi: 10.1364/ol.30.002272.

Authors

Benjamin A Flusberg¹, Juergen C Jung, Eric D Cocker, Erik P Anderson, Mark J Schnitzer

Affiliation

¹ James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305-5435, USA.

PMID: 16190441
DOI: 10.1364/ol.30.002272

Abstract

We introduce a compact two-photon fluorescence microendoscope based on a compound gradient refractive index endoscope probe, a DC micromotor for remote adjustment of the image plane, and a flexible photonic bandgap fiber for near distortion-free delivery of ultrashort excitation pulses. The imaging head has a mass of only 3.9 g and provides micrometer-scale resolution. We used portable two-photon microendoscopy to visualize hippocampal blood vessels in the brains of live mice.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Endoscopes*
Equipment Design
Equipment Failure Analysis
Hippocampus / blood supply*
Hippocampus / cytology*
Image Enhancement / instrumentation*
Image Enhancement / methods
Mice
Microscopy, Fluorescence, Multiphoton / instrumentation*
Microscopy, Fluorescence, Multiphoton / methods
Miniaturization