Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source

D M Grant; D S Elson; D Schimpf; C Dunsby; J Requejo-Isidro; E Auksorius; I Munro; M A A Neil; P M W French; E Nye; G Stamp; P Courtney

doi:10.1364/ol.30.003353

Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source

Opt Lett. 2005 Dec 15;30(24):3353-5. doi: 10.1364/ol.30.003353.

Authors

D M Grant¹, D S Elson, D Schimpf, C Dunsby, J Requejo-Isidro, E Auksorius, I Munro, M A A Neil, P M W French, E Nye, G Stamp, P Courtney

Affiliation

¹ Physics Department, Imperial College London, Prince Consort Road, London SW7 2BW, UK.

PMID: 16389829
DOI: 10.1364/ol.30.003353

Abstract

We demonstrate an optically sectioned fluorescence lifetime imaging microscope with a wide-field detector, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber.