Abstract
We describe the isolation of seven different protein-tyrosine phosphatase (PTPase) cDNAs from Drosophila embryos, three of which are primarily expressed in the central nervous system (CNS). The CNS-specific PTPases include the previously sequenced DLAR, as well as two novel PTPases (denoted DPTP10D and DPTP99A), which have extracellular domains consisting of multiple fibronectin type III repeats. Each of the Drosophila sequences is most closely related to a different human PTPase. The three PTPase mRNAs are expressed in different patterns of cells in the ventral nerve cord, and all three proteins are restricted to axons. DLAR and DPTP99A are apparently expressed on most or all axons, while DPTP10D is primarily localized to the anterior commissure and its junctions with the longitudinal tracts.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Axons / physiology*
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cell Adhesion Molecules
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Central Nervous System / embryology*
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DNA / genetics
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Drosophila melanogaster / embryology*
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Drosophila melanogaster / genetics
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Gene Expression
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Immunoenzyme Techniques
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Tyrosine Phosphatases / genetics*
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RNA, Messenger / genetics
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Receptors, Cell Surface / physiology*
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Restriction Mapping
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Sequence Alignment
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Signal Transduction
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Transcription, Genetic
Substances
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Cell Adhesion Molecules
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RNA, Messenger
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Receptors, Cell Surface
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DNA
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Protein Tyrosine Phosphatases
Associated data
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GENBANK/M80538
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GENBANK/M80539
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GENBANK/S60911
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GENBANK/S60914
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GENBANK/S60917
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GENBANK/S60919
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GENBANK/S60920
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GENBANK/S60969
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GENBANK/S61522
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GENBANK/S61524