Abstract
Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids / chemistry
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Amino Acids / metabolism
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Oligopeptides / chemical synthesis*
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Protein Engineering / methods*
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Transfer / chemistry*
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RNA, Transfer / genetics
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RNA, Transfer / metabolism
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Transfer RNA Aminoacylation*
Substances
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Amino Acids
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Oligopeptides
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RNA, Messenger
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RNA, Transfer