Abstract
Angiomotin, an 80 kDa protein expressed in endothelial cells, promotes cell migration and invasion, and stabilizes tube formation in vitro. Angiomotin belongs to a new protein family with two additional members, Amotl-1 and Amotl-2, which are characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. Here, we report the identification of a 130 kDa splice isoform of angiomotin that is expressed in different cell types including vascular endothelial cells, as well as cytotrophoblasts of the placenta. p130-Angiomotin consists of a cytoplasmic N-terminal extension that mediates its association with F-actin. Transfection of p130-angiomotin into endothelial cells induces actin fiber formation and changes cell shape. The p130-angiomotin protein remained associated with actin after destabilization of actin fibers with cytochalasin B. In contrast to p80-angiomotin, p130-angiomotin does not promote cell migration and did not respond to angiostatin. We propose that p80- and p130-angiomotin play coordinating roles in tube formation by affecting cell migration and cell shape, respectively.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism*
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Alternative Splicing
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Amino Acid Sequence
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Angiomotins
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Angiostatins / physiology
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Animals
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Cell Line
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Cell Line, Transformed
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Cell Movement / physiology
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Cell Shape / physiology*
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Endothelial Cells / cytology*
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Endothelial Cells / metabolism*
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Endothelium, Vascular / cytology
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Endothelium, Vascular / metabolism
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HeLa Cells
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Humans
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Intercellular Signaling Peptides and Proteins / biosynthesis
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Intercellular Signaling Peptides and Proteins / physiology
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Membrane Proteins / physiology
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Mice
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Mice, Knockout
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Microfilament Proteins / biosynthesis
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism
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Microfilament Proteins / physiology
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Molecular Sequence Data
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Protein Isoforms / physiology
Substances
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AMOT protein, human
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Actins
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Amot protein, mouse
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Angiomotins
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins
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Microfilament Proteins
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Protein Isoforms
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Angiostatins