FREALIGN: high-resolution refinement of single particle structures

Nikolaus Grigorieff

doi:10.1016/j.jsb.2006.05.004

FREALIGN: high-resolution refinement of single particle structures

J Struct Biol. 2007 Jan;157(1):117-25. doi: 10.1016/j.jsb.2006.05.004. Epub 2006 Jun 2.

Author

Nikolaus Grigorieff¹

Affiliation

¹ Howard Hughes Medical Institute, Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA. niko@brandeis.edu

PMID: 16828314
DOI: 10.1016/j.jsb.2006.05.004

Abstract

The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Image Enhancement / methods*
Imaging, Three-Dimensional / methods*
Microscopy, Electron
Particle Size
Software Design
Software*

Grants and funding

1 P01 GM-62580/GM/NIGMS NIH HHS/United States